Determination of Albumin in Bronchoalveolar Lavage Fluid by Flow-Injection Fluorometry Using Chromazurol S(Analytical Biochemistry)
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概要
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A highly sensitive flow injection fluorometry for the determination of albumin was developed and applied to the determination of albumin in human bronchoalveolar lavage fluids (BALF). This method is based on binding of chromazurol S (CAS) to albumin. The calibration curve was linear in the range of 5-200 μg/ml of albumin. A highly linear correlation (r=0.986) was observed between the albumin level in BALF samples (n=25) determined by the proposed method and by a conventional fluorometric method using CAS (CAS manual method). The IgG interference was lower in the CAS flow injection method than in the CAS manual method. The albumin level in BALF collected from healthy volunteers (n=10) was 58.5±13.1 μg/ml. The albumin levels in BALF samples obtained from patients with sarcoidosis and idiopathic pulmonary fibrosis were increased. This finding shows that the determination of albumin levels in BALF samples is useful for investigating lung diseases and that CAS flow injection method is promising in the determination of trace albumin in BALF samples, because it is sensitive and precise.
- 公益社団法人日本薬学会の論文
- 2008-03-01
著者
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Saito Yutaka
Department Of Pharmaceutical Sciences Graduate School Of Medicine And Dentistry And Pharmaceutical S
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NAGAI Sonoko
Department of Respiratory Medicine, Graduate School of Medicine, Kyoto University
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Nagai Sonoko
Department Of Respiratory Medicine Graduate School Of Medicine Kyoto University
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Saito Yoshihiro
Department Of Bio-analytical Chemistry Osaka University Of Pharmaceutical Sciences
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CHIKUMA Masahiko
Osaka University of Pharmaceutical Sciences
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SAITO Yoshihiro
Laboratory of Bio-analytical Chemistry, Osaka University of Pharmaceutical Sciences
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SATO Takaji
Laboratory of Bio-analytical Chemistry, Osaka University of Pharmaceutical Sciences
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CHIKUMA Masahiko
Laboratory of Bio-analytical Chemistry, Osaka University of Pharmaceutical Sciences
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SATO Takaji
Osaka University of Pharmaceutical Sciences
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SAITO Yoshihiro
Osaka University of Pharmaceutical Sciences
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Sato Takaji
Department Of Bio-analytical Chemistry Osaka University Of Pharmaceutical Sciences
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齊戸 美弘
Department Of Ophthalmology Osaka National Hospital
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Saito Yoshihiro
Laboratory Of Bio-analytical Chemistry Osaka University Of Pharmaceutical Sciences
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Saito Yutaka
Department Of Endoscopy National Cancer Center Hospital
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Saito Yutaka
Osaka University Of Pharmaceutical Sciences:faculty Of Pharmaceutical Sciences Okayama University:ch
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Chikuma Masahiko
Osaka College of Pharmacy
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Saito Yutaka
Department of Chemistry, Nagaoka University of Technology
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