Reverse Transfection Using Antibodies against a Cell Surface Antigen in Mammalian Adherent Cell Lines(CELL AND TISSUE ENGINEERING)
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概要
- 論文の詳細を見る
Reverse transfection from a solid surface has the potential to deliver genes to various cells more efficiently than conventional methods. However, the effective gene delivery from a solid surface requires an optimized extracellular matrix (ECM) for the coating of glass slides, dependent on the nature of the cells. In a search for an appropriate substrate for the universal application to multiple types of cell, we focused on cell surface antigens and examined the effects of antibodies raised against them on gene transfer from an antibody-coated surface. We found that a coating of CD29-specific antibody allowed the most effective delivery of genes by reverse transfection in every type of cell that we examined. Our results suggest that reverse transfection with antibodies against CD29 might provide a universal tool for gene delivery and cell array-based analyses.
- 社団法人日本生物工学会の論文
- 2007-08-25
著者
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Miyake Jun
Research Institute of Cell Engineering, National Institute of Advanced Industrial Science and Techno
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Fujita Satoshi
Research Institute For Cell Engineering (rice) National Institute Of Advanced Industrial Science And
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Fujita Satoshi
Department Of Bioengineering School Of Engineering The University Of Tokyo:research Institute For Ce
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YAMADA Shigeru
Research Center Hospital for Charged Particle Therapy, National Institute of Radiological Sciences
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Miyake Masato
Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science an
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Uchimura Eiichiro
Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science an
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Nomura Takashi
Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science an
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Matsumoto Kayo
Research Institute for Cell Engineering(RICE), National Institute of Advanced Industrial Science and
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Miyake Masato
Research Institute For Cell Engineering (rice) National Institute Of Advanced Industrial Science And
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Yamada Shigeru
Research Institute For Cell Engineering (rice) National Institute Of Advanced Industrial Science And
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Miyake Jun
Department Of Bioengineering School Of Engineering The University Of Tokyo:research Institute For Ce
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Matsumoto Kayo
Research Institute For Cell Engineering(rice) National Institute Of Advanced Industrial Science And
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Uchimura Eiichiro
Research Institute For Cell Engineering (rice) National Institute Of Advanced Industrial Science And
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Fujita Satoshi
Department Of Bioengineering School Of Engineering The University Of Tokyo:research Institute For Ce
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Miyake Masato
Research Institute For Cell Engineering (rice) National Institute Of Advanced Industrial Science And
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Miyake Jun
Research Institute For Cell Engineering National Institute Of Advanced Industrial Science And Techno
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Yamada Shigeru
Research Center For Charged Particle Therapy National Institute Of Radiological Sciences
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Yamada Shigeru
Research Center For Advanced Science And Technology The University Of Tokyo
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Nomura Takashi
Research Institute For Cell Engineering (rice) National Institute Of Advanced Industrial Science And
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Miyake Jun
Research Institute For Cell Engineering (rice) National Institute Of Advanced Industrial Science And
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