Highly Efficient Reverse Transfection with siRNA in Multiple Wells of Microtiter Plates(METHODS)
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概要
- 論文の詳細を見る
We have developed an efficient and inexpensive method of reverse transfection from the solid phase to suppress genes with siRNA. The method enabled the realization of (i) a high efficiency of transfection ; (ii) transfection of various types of cell ; (iii) a high efficiency of gene knockdown by siRNA ; (iv) a low toxicity to cells ; and (v) a long-term stabilization (more than 210 d) of attached transfection mixture including siRNA in multiple wells. Although array-based reverse transfection has advantages in terms of miniaturization, the method has the advantage of enabling the inclusion of various soluble factors, such as humoral factors, drugs and ligands that affect gene expression, because the liquid phase is partitioned within the individual wells of each microtiter plate. Our method of reverse transfection with siRNA in multiple wells is a powerful and high-through-put tool for the analysis of signaling pathways.
- 社団法人日本生物工学会の論文
- 2007-10-25
著者
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Miyake Jun
Research Institute of Cell Engineering, National Institute of Advanced Industrial Science and Techno
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Fujita Satoshi
Research Institute For Cell Engineering (rice) National Institute Of Advanced Industrial Science And
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Fujita Satoshi
Department Of Bioengineering School Of Engineering The University Of Tokyo:research Institute For Ce
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Ota Eiji
Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science an
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Sasaki Chie
Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science an
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Takano Kota
Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science an
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Miyake Masato
Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science an
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Miyake Masato
Research Institute For Cell Engineering (rice) National Institute Of Advanced Industrial Science And
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Miyake Jun
Department Of Bioengineering School Of Engineering The University Of Tokyo:research Institute For Ce
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Ota Eiji
Research Institute For Cell Engineering (rice) National Institute Of Advanced Industrial Science And
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Sasaki Chie
Research Institute For Cell Engineering (rice) National Institute Of Advanced Industrial Science And
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Takano Kota
Research Institute For Cell Engineering (rice) National Institute Of Advanced Industrial Science And
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Fujita Satoshi
Department Of Bioengineering School Of Engineering The University Of Tokyo:research Institute For Ce
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Miyake Masato
Research Institute For Cell Engineering (rice) National Institute Of Advanced Industrial Science And
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Miyake Jun
Research Institute For Cell Engineering National Institute Of Advanced Industrial Science And Techno
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Miyake Jun
Research Institute For Cell Engineering (rice) National Institute Of Advanced Industrial Science And
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