Onset timing of transient gene expression depends on cell division(CELL AND TISSUE ENGINEERING)
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概要
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By using the time lapse of both phase-contrast and fluorescent images, we examined the morphology of cells and the dynamics of gene expression (EGFP). We applied k-means clustering to the time course data of fluorescent intensity of EGFP and successfully found four subpopulations. Discriminating the appropriate clusters and investigating the details of them, we found that almost all cells express the transfected gene after cell division and also found there is a strong correlation between onset timing and cell division. This result suggested that it is possible to normalize the dynamics of gene by arranging the onset timing of gene expression or by arranging the cell division.
- 社団法人日本生物工学会の論文
著者
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Fujita Satoshi
Department of Emergency Medicine, Asahikawa Medical College
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Miyake Jun
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo
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Fujita Satoshi
Research Institute For Cell Engineering (rice) National Institute Of Advanced Industrial Science And
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Fujita Satoshi
Department Of Bioengineering School Of Engineering The University Of Tokyo:research Institute For Ce
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HAKAMADA KAZUMI
Department of Biotechnology, School of Engineering, Nagoya University
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Hakamada Kazumi
Department Of Bioengineering School Of Engineering The University Of Tokyo:research Institute For Ce
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Hakamada Kazumi
Department Of Biotechnology School Of Engineering Nagoya University:(present Address)laboratory For
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Miyake Jun
Department Of Bioengineering Graduate School Of Engineering The University Of Tokyo:research Institu
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Miyake Jun
Department Of Bioengineering School Of Engineering The University Of Tokyo:research Institute For Ce
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Fujita Satoshi
Department Of Bioengineering School Of Engineering The University Of Tokyo:research Institute For Ce
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Miyake Jun
Department Of Bioengineering Graduate School Of Engineering The University Of Tokyo:department Of Me
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Fujita Satoshi
Department Of Anesthesiology School Of Medicine Sapporo Medical University
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Miyake Jun
Department Of Bioengineering Graduate School Of Engineering The University Of Tokyo
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