Enhanced Hydrogen Production by a Mutant of Rhodobacter sphaeroides Having an Altered Light-Harvesting System

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概要

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• A stable mutant of the photosynthetic bacterium Rhodobacter sphaeroides with an altered light-harvesting(LH)system(P3 mutant)was obtained by UV irradiation and characterized. The mutant exhibited a 2.7-fold decrease in the core antennal(LH1)content and 1.6-fold increase in peripheral antennal(LH2)content compared to the wild-type strain. The H_2 evolution rates in the P3 mutant under 800- and 850-nm light, corresponding to the absorption maxima of LH2, were 1.5 times higher than in the wild-type strain. The wild-type absorption spectrum was restored in the P3 mutant when a 1.1-kb PCR-amplified fragment containing the puf promoter and pufQBA genes was ligated into a pRK-415 derivative and introduced into it. The transformant showed lower H_2 production rates at 800 and 850 nm than the P3 strain carrying the control plasmid, indicating that the accelerated H_2 production in the P3 mutant was a result of alterations in the LH system.

• 社団法人日本生物工学会の論文
• 1999-05-25

著者

• Hara M

  Tissue Engineering Research Center National Institute Of Advanced Industrial Science And Technology

• Miyake J

  Dep. Of Bioengineering Graduate School Of Engineering The Univ. Of Tokyo 7-3-1 Hongo Bunkyo-ku Tokyo

• Miyake Masato

  Miti:fuji Electric Corpotate R&d Ltd.:(present Address)institute Of Soil Science And Photosynthe

• WAKAYAMA TATSUKI

  Tissue Engineering Research Center, National Institute of Advanced Industrial Science and Technology

• MIYAKE Jun

  National Institute for Advanced Interdisciplinary Research, Agency of Industrial Science and Technol

• ASADA YASUO

  National Institute of Biosciencd and Human-Technology

• HARA MASAYUKI

  National Institute for Advanced Interdisciplinary Research, Agency of Industrial Science and Technol

• VASILYEVA LYUDMILA

  Research Institute of Innovative Technology for the Earth, NIBH Laboratory, AIST

• KHATIPOV EMIR

  National Institute of Bioscience and Human-Technology, AIST

• WAKAYAMA TATSUKI

  MITI

• SEKINE MAKOTO

  Research Institute of Innovative Technology for the Earth, NIBH Laboratory, AIST

• HARA MASAYUKI

  MITI

• NAKADA EIJU

  MITI

• ASADA YASUO

  Research Institute of Innovative Technology for the Earth, NIBH Laboratory, AIST

• MIYAKE JUN

  MITI

• Miyake J

  Fermentation Research Inst. Ibaraki Jpn

• Miyake Jun

  Department Of Bioengineering Graduate School Of Engineering The University Of Tokyo:research Institu

• Miyake Jun

  Department Of Mechanical Science And Bioengineering Graduate School Of Engineering Science Osaka Uni

• Miyake J

  Tissue Engineering Research Center (terc) National Institute Of Advanced Industrial Science And Tech

• Shirai M

  Ibaraki Univ. Ibaraki Jpn

• Asada Y

  National Institute Of Bioscience And Human-technology Aist Miti

• Khatipov Emir

  National Institute Of Bioscience And Human-technology Aist

• Miyake M

  Miti:fuji Electric Corpotate R&d Ltd.:(present Address)institute Of Soil Science And Photosynthe

• MIYAKE Masato

  National Institute of Bioscience and Human-Technology

• Miyake J

  Tokyo Univ. Agric. & Technol.:rice Aist

• NAKADA Eiju

  Research Institute of Innovative Technology for the Earth (at NIBH)

• Vasilyeva Lyudmila

  Research Institute Of Innovative Technology For The Earth Nibh Laboratory Aist

• KHATIPOV EMIR

  Research Institute of Innovative Technology for the Earth, NIBH Laboratory, AIST/MITI

• WAKAYAMA TATSUKI

  Research Institute of Innovative Technology for the Earth, NIBH Laboratory, AIST/MITI

• SEKINE MAKOTO

  National Institute of Bioscience and Human-Technology, AIST/MITI

• Khatipov Emir

  Research Institute Of Innovative Technology For The Earth Nibh Laboratory Aist/miti:national Institu

• Asada Yasuo

  National Institute Of Bioscience And Human-technology

• Wakayama Tatsuki

  Tissue Engineering Research Center National Institute Of Advanced Industrial Science And Technology

• Miyake Masato

  National Institute Of Bioscience And Human Technology Agency Of Industrial Science And Technology Mi

• Miyake Jun

  National Institute For Advanced Interdisciplinary Research Aist

• Miyake Jun

  National Institute Of Bioscience And Human-technology

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