Enhancement of Hydrogen Production by a Photosynthetic Bacterium Mutant with Reduced Pigment
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概要
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A novel mutant MTP4 was created from the wild-type strain Rhodobacter sphaeroides RV by UV irradiation for the enhancement of hydrogen production. The amount of light absorbed by MTP4 was lower than that by the wild-type strain at any wavelengths ranging from 350 to 1000 nm. This nature enables the illumination of cells in the deeper parts of a reactor. The contents of bacteriochlorophylls and carotenoids of the chromatophores prepared from MTP4 under the conditions for hydrogen production were reduced to 41 and 49% of those from the wild-type strain RV, respectively. Analysis of the light-harvesting (LH) complexes by SDS-PAGE showed that the amounts of LH1s and reaction centers (RCs) in MTP4 were retained, whereas that of LH2s was much less than that in RV. Although MTP4 had less pigments, its growth rate was equivalent to that of RV over a wide range of light intensities. MTP4 produces hydrogen with a stable manner. Using a plate-type reactor, it produced 50% more hydrogen than RV. A novel method of pigment reduction was found to be effective for the enhancement of hydrogen production per unit reactor.
- 社団法人日本生物工学会の論文
- 2002-02-25
著者
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Hara M
Tissue Engineering Research Center National Institute Of Advanced Industrial Science And Technology
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Miyake J
Dep. Of Bioengineering Graduate School Of Engineering The Univ. Of Tokyo 7-3-1 Hongo Bunkyo-ku Tokyo
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MIYAKE Jun
Tissue Engineering Research Center, AIST
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Kondo Toshihiko
Ntt Telecommunications Energy Laboratories Ntt Corporation
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ARAKAWA MASAYASU
NTT Telecommunications Energy Laboratories, NTT Corporation
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HIRAI TOSHIRO
NTT Telecommunications Energy Laboratories, NTT Corporation
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WAKAYAMA TATSUKI
Tissue Engineering Research Center, National Institute of Advanced Industrial Science and Technology
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HARA MASAYUKI
Tissue Engineering Research Center, National Institute of Advanced Industrial Science and Technology
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Hara Masayuki
Tissue Engineering Research Center National Institute Of Advanced Industrial Science And Technology
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Miyake J
Fermentation Research Inst. Ibaraki Jpn
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Miyake Jun
Department Of Bioengineering Graduate School Of Engineering The University Of Tokyo:research Institu
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Miyake Jun
Department Of Mechanical Science And Bioengineering Graduate School Of Engineering Science Osaka Uni
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Miyake J
Tissue Engineering Research Center (terc) National Institute Of Advanced Industrial Science And Tech
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Hirai Toshiro
Ntt Telecommunications Energy Laboratories Ntt Corporation:(present Address)ennet Corporation
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Miyake J
Tokyo Univ. Agric. & Technol.:rice Aist
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Arakawa Masayasu
Ntt Telecommunications Energy Laboratories Ntt Corporation
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Wakayama Tatsuki
Tissue Engineering Research Center National Institute Of Advanced Industrial Science And Technology
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Miyake Jun
Tissue Engineering Research Center Aist
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Miyake Jun
National Institute Of Bioscience And Human-technology
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