Measurement of Intramolecular Energy Dissipation and Stiffness of a Single Peptide Molecule by Magnetically Modulated Atomic Force Microscopy
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概要
- 論文の詳細を見る
A method for measuring intramolecular energy dissipation as well as stiffness variation in a single biomolecule in situ by atomic force microscopy (AFM) is presented. An AFM cantilever is magnetically modulated at an off-resonance frequency while it elongates a single peptide molecule in buffer solution. The molecular stiffness and the energy dissipation are measured via the amplitude and phase lag in the response signal. Data showing a peculiar feature in both profiles of stiffness and dissipation is presented. This suggests that the present method is more sensitive to the state of the molecule than the conventional force-elongation measurement is.
- Japan Society of Applied Physicsの論文
- 2004-12-01
著者
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NAKAMURA Chikashi
Tissue Engineering Research Center, National Institute of Advanced Industrial Science and Technology
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KAGESHIMA Masami
Nanotechnology Research Institute, National Institute of Advanced Industrial Science and Technology
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TAKEDA Seiji
Tissue Engineering Research Center, National Institute of Advanced Industrial Science and Technology
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PTAK Arkadiusz
Poznan University of Technology, Institute of Physics
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JARVIS Suzanne
Nanotechnology Research Institute, National Institute of Advanced Industrial Science and Technology
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Tokumoto Hiroshi
Nanotechnology Research Center Research Institute For Electronic Science Hokkaido University
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Miyake Jun
Tissue Engineering Research Center Aist
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Nakamura Chikashi
Tissue Engineering Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-46 Nakoji, Amagasaki, Hyogo 661-0974, Japan
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Takeda Seiji
Tissue Engineering Research Center (TERC), National Institute of Advanced Industrial Science and Technology (AIST)
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Tokumoto Hiroshi
Nanotechnology Research Institute, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan
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Kageshima Masami
Nanotechnology Research Institute, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan
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Ptak Arkadiusz
Poznan University of Technology, Institute of Physics, Nieszawska 13 A, 60-965 Poznan, Poland
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Takeda Seiji
Tissue Engineering Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-46 Nakoji, Amagasaki, Hyogo 661-0974, Japan
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