Power-Law Stress and Creep Relaxations of Single Cells Measured by Colloidal Probe Atomic Force Microscopy
スポンサーリンク
概要
- 論文の詳細を見る
We measured stress and creep relaxations of mouse fibroblast cells arranged and cultured on a microarray, by colloidal probe atomic force microscopy (AFM). A hydrophobic monolayer coating of perfluorodecyltrichlorosilane (FDTS) on the surface of colloidal silica beads significantly reduced the adhesion force of live cells, compared with untreated beads. The rheological behaviors of cells were estimated by averaging several relaxation curves of cells measured by the AFM. Longer-time tailing of both stress and creep relaxation curves followed single power-law behavior over a time scale of 60 s, with exponents in the range 0.1–0.4, varying with cells. The results were in good agreement with previous measurements of the frequency-domain rheology of cells using the force modulation mode.
- Japan Society of Applied Physicsの論文
- 2009-08-25
著者
-
Mizutani Yusuke
Graduate School Of Information Science And Technology Hokkaido University
-
Hiratsuka Shinichiro
Graduate School Of Information Science And Technology Hokkaido University
-
Okajima Takaharu
Graduate School Of Information Science And Technology Hokkaido University
-
Tokumoto Hiroshi
Nanotechnology Research Center Research Institute For Electronic Science Hokkaido University
-
Kawahara Koichi
Graduate School Of Information Science And Technology Hokkaido University
-
Mizutani Yusuke
Graduate School of Information Science and Technology, Hokkaido University, Sapporo 060-0814, Japan
-
Toda Akitoshi
MEMS Technology Division, Olympus Corporation, Hachioji, Tokyo 192-8512, Japan
-
Fukushima Norichika
MEMS Technology Division, Olympus Corporation, Hachioji, Tokyo 192-8512, Japan
関連論文
- 3P-308 原子間力顕微鏡・蛍光相関分光法を用いた細胞膜表面の局所領域測定(計測,第46回日本生物物理学会年会)
- Study of Field Emission Characteristics of Carbon with the Scanning Atom Probe
- 3P-157 周期構造を有する細胞シートを用いた細胞力学(細胞生物的課題(接着,運動,骨格,伝達,膜),第47回日本生物物理学会年会)
- 2P-209 細胞間相互作用の測定のための生細胞配列構造体の開発(細胞生物学的課題(2),第46回日本生物物理学会年会)
- Behavior of Catalyst Particle at Tip of Carbon Nanotube during Field Emission
- Direct Observation of Field Emission Sites in a Single Multiwalled Carbon Nanotube by Lorenz Microscopy
- 3P192 細胞シート一軸延伸法による細胞メカニクスの研究(細胞生物的課題(接着,運動骨格,伝達,膜),第48回日本生物物理学会年会)
- 1P322 原子間力顕微鏡を用いた形質転換過程における線維芽細胞のレオロジー特性評価(計測,第48回日本生物物理学会年会)
- 3P-246 原子間力顕微鏡を用いた酸化ストレスによる細胞肥大過程の力学特性評価(計測,第47回日本生物物理学会年会)
- 3P-314 マイクロアレイを用いたAFMによる多数生細胞の力学計測(計測,第46回日本生物物理学会年会)
- 3P-311 原子間力顕微鏡を用いた多数細胞粘弾性の精密測定(計測,第46回日本生物物理学会年会)
- Elasticity of Living Cells on a Microarray during the Early Stages of Adhesion Measured by Atomic Force Microscopy
- Rheological Properties of Growth-Arrested Fibroblast Cells under Serum Starvation Measured by Atomic Force Microscopy
- Rheological properties of growth-arrested fibroblast cells under serum starvation measured by atomic force microscopy (Special issue: Scanning probe microscopy)
- Distinct Chemical Contrast in Adhesion Force Images of Hydrophobic–Hydrophilic Patterned Surfaces Using Multiwalled Carbon Nanotube Probe Tips
- Direct observation of dynamic force propagation between focal adhesions of cells on microposts by atomic force microscopy
- 1J1324 原子間力顕微鏡を用いた正常細胞とがん細胞の粘弾性測定(計測,第49回日本生物物理学会年会)
- 3K1112 原子間力顕微鏡を用いた水平方向の力学刺激による細胞内カルシウム応答測定(細胞生物的課題3,第49回年会講演予稿集)
- 3K1124 応力緩和測定における細胞シートポアソン比の時間依存性(細胞生物的課題3,第49回年会講演予稿集)
- Stress Relaxation Measurement of Fibroblast Cells with Atomic Force Microscopy
- 3PS014 細胞シート1軸延伸測定法による時間依存力学測定(日本生物物理学会第50回年会(2012年度))
- Power-Law Stress and Creep Relaxations of Single Cells Measured by Colloidal Probe Atomic Force Microscopy
- Measurement of Intramolecular Energy Dissipation and Stiffness of a Single Peptide Molecule by Magnetically Modulated Atomic Force Microscopy
- Energy Spread of Field Emission Electrons from Single Pentagons in Individual Multi-Walled Carbon Nanotubes
- Field Emission Stability of Individual Multi-Walled Carbon Nanotubes
- Stress Relaxation Measurement of Fibroblast Cells with Atomic Force Microscopy
- 3PS015 原子間力顕微鏡を用いた単一細胞レオロジーの高速・精密測定法の開発(日本生物物理学会第50回年会(2012年度))
- Elasticity of Living Cells on a Microarray during the Early Stages of Adhesion Measured by Atomic Force Microscopy
- Versatility of Self-Oscillation Technique with Mechanical-Acoustic Excitations for Frequency Modulation Atomic Force Microscope in Liquids
- Scanning Tunneling Microscopy Observation of Apparent Molecular Motion Induced by Polarity Change of Electric Fields
- Power-Law Stress and Creep Relaxations of Single Cells Measured by Colloidal Probe Atomic Force Microscopy
- 2P213 単一細胞膜揺らぎ計測のためのイオンコンダクタンス顕微鏡技術の開発(13B.生体膜・人工膜:ダイナミクス,ポスター,日本生物物理学会年会第51回(2013年度))