Direct observation of dynamic force propagation between focal adhesions of cells on microposts by atomic force microscopy
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概要
- 論文の詳細を見る
We investigated dynamic force propagation between focal adhesions of fibroblast cells cultured on polydimethylsiloxane micropost substrates, by atomic force microscopy. Live cells were mechanically modulated by the atomic force microscopy probe bound to cell apical surfaces at 0.01-0.5 Hz, while microposts served as a force sensor at basal surfaces. We observed that cells exhibited rheological behavior at the apical surface but had no apparent out-of-phase response at the basal surface, indicating that the dynamic force propagating through cytoskeletal filaments behaves in an elastic manner. Moreover, the direction of the propagated force was observed to be intimately associated with the prestress.
- 2011-12-26
著者
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Sueoka Kazuhisa
Nanoelectronics Laboratory Graduate School Of Engineering Hokkaido University
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Mizutani Yusuke
Graduate School Of Information Science And Technology Hokkaido University
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Kawahara Koichi
Graduate School Of Information Science And Technology Hokkaido University
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Kawahara Kouichi
Graduate School Of Information Science And Technology Hokkaido University
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Mizutani Yusuke
Grad. Sch. Info. Sci. & Tech. Hokkaido. Univ.
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Okajima Takaharu
Grad. Sch. Info. Sci & Tech Hokkaido Univ.
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