Rheological Properties of Growth-Arrested Fibroblast Cells under Serum Starvation Measured by Atomic Force Microscopy
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概要
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The rheological properties of growth-arrested and quiescent (G0 phase) mousefibroblast cells under serum starvation were investigated by atomic force microscopy(AFM) with a microarray technique. The number distribution of complex shear modulus,G*, of quiescent cells at the serum concentration, CS = 0.1%, followed a log-normaldistribution, and the frequency dependence of G* exhibited a power law behavior,which were similar to those under a control condition at CS = 10%. On the other hand,we found that the Newtonian viscosity coefficient of the quiescent cells significantlyincreased, and the distribution broadened, as compared with the control cells, whereasthe power-law exponent was unchanged. The result indicated that the rheologicalproperties of quiescent fibroblast cells were not identical to those in the G1 phaseduring cell cycle. This finding suggests that the Newtonian viscosity of cells is one ofthe useful indicators for evaluating growth-arrested cells under serum starvation.
著者
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Mizutani Yusuke
Graduate School Of Information Science And Technology Hokkaido University
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Tsuchiya Masahiro
Graduate School Of Information Science And Technology Hokkaido University
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Okajima Takaharu
Graduate School Of Information Science And Technology Hokkaido University
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Kawahara Koichi
Graduate School Of Information Science And Technology Hokkaido University
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Kawahara Kouichi
Graduate School Of Information Science And Technology Hokkaido University
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Okajima Takaharu
Graduate School of Information Science and Technology, Hokkaido University, Sapporo 060-0814, Japan
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Miyaoka Atsushi
Graduate School of Information Science and Technology, Hokkaido University, Sapporo 060-0814, Japan
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