Stress Relaxation Measurement of Fibroblast Cells with Atomic Force Microscopy

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概要

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• We measured the stress relaxation of mouse fibroblast NIH3T3 cells with an atomic force microscope (AFM) using a sharp silicon tip and a silica bead with a radius of ∼1 µm as an indenter. The decay of loading force was clearly observed in NIH3T3 cells at a small initial loading force of ∼0.4 nN and was well fitted to the stretched exponential function rather than to a single exponential function. The stretching exponent parameter was ∼0.5 for both indenters, indicating that the stress relaxation observed in NIH3T3 cells consisted of multiple relaxation processes. The time-domain AFM technique described in this report allows us to measure directly the relaxation process of living cells in a range from milliseconds to seconds.

• 2007-08-30

著者

• Shimomura Masatsugu

  Institute Of Multidisciplinary Research For Advanced Materials (imram) Tohoku University

• Okajima Takaharu

  Graduate School Of Information Science And Technology Hokkaido University

• Tokumoto Hiroshi

  Nanotechnology Research Center Research Institute For Electronic Science Hokkaido University

• Tanaka Masaru

  Institute Of Pharmaceutical Sciences Faculty Of Medicine Hiroshima University

• Tsukiyama Shusaku

  Creative Research Initiative "Sousei", Hokkaido University, N21 W10, Kita-ku, Sapporo 001-0021, Japan

• Kadowaki Tsubasa

  Graduate School of Information Science and Technology, Hokkaido University, Sapporo 060-0814, Japan

• Yamamoto Sadaaki

  Creative Research Initiative, "Sousei", Hokkaido University, Sapporo 001-0021, Japan

• Yamamoto Sadaaki

  Creative Research Initiative "Sousei", Hokkaido University, N21 W10, Kita-ku, Sapporo 001-0021, Japan

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