Enhanced Cell Survival and Yield of Rat Small Hepatocytes by Honeycomb-Patterned Films

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概要

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• Surface designing of substrate to regulate cell adhesion and function in nano and micro scale is a critical issue in biomaterial science. In this study, we describe the fabrication of highly regular patterned porous films (honeycomb-patterned film) formed by a simply casting technique, and the culture of mature hepatocytes and small hepatocytes on the films. The pore size of the honeycomb-patterned films used was 6, 12, and 16 μm. We evaluated the effect of the honeycomb-patterned films on the morphology, cell yield, survival and the differentiated hepatic function (albumin production) of the both hepatocytes. Both hepatocytes attached on the flat films appeared to spread well, showing a typical monolayer morphology. They peeled off from the films at 7 days in culture on the flat films. On the other hand, spreading of the each hepatocytes was restricted on the honeycomb-patterned films at 3 and 7 days in culture. The cell yield and survival of the each hepatocytes increased with increasing culture time. Small hepatocyte on the pore sizes of 16 μm showed the highest cell yield (approximately 3 times). Albumin production of mature hepatocyte on the pore sizes of 16 μm ($224.1.3 \pm 157$ ng ml-1 well-1 at 1 day in culture, $369.5 \pm 222$ ng ml-1 well-1 at 3 days in culture) was higher than that of the hepatocytes on the flat films ($119.3 \pm 9.3$ ng ml-1 well-1 at 1 day in culture, $262.8 \pm 47.3$ ng ml-1 well-1 at 3 days in culture), although that of small hepatocytes on the honeycomb-patterned films (pore size: 16 μm) was similar on the flat film. These results indicated that both the surface topography and the pore size of the honeycomb-patterned film affected the hepatic metabolic function.

• Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
• 2008-02-25

著者

• Shimomura Masatsugu

  Institute Of Multidisciplinary Research For Advanced Materials (imram) Tohoku University

• Todo Satoru

  First Department Of Surgery Faculty Of Medicine Hokkaido University

• Tanaka Masaru

  Institute Of Pharmaceutical Sciences Faculty Of Medicine Hiroshima University

• Tsukiyama Shusaku

  Creative Research Initiative "Sousei", Hokkaido University, N21 W10, Kita-ku, Sapporo 001-0021, Japan

• Matsushita Michiaki

  First Department of Surgery, Hokkaido University Graduate School of Medicine, N14 W5, Kita-ku, Sapporo 060-8648, Japan

• Yamamoto Sadaaki

  Creative Research Initiative "Sousei", Hokkaido University, N21 W10, Kita-ku, Sapporo 001-0021, Japan

• Tamura Hitoshi

  First Department of Surgery, Hokkaido University Graduate School of Medicine, N14 W5, Kita-ku, Sapporo 060-8648, Japan

• Todo Satoru

  First Department of Surgery, Hokkaido University Graduate School of Medicine, N14 W5, Kita-ku, Sapporo 060-8648, Japan

• Shimomura Masatsugu

  Institute of Multidisciplinary Research for Advanced Materials (IMRAM), Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan

• Tanaka Masaru

  Institute of Multidisciplinary Research for Advanced Materials (IMRAM), Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan

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