Entrapment of Rhodobacter sphaeroides RV in Cationic polymer/Agar Gels for Hydrogen Production in the Presence of NH^+_4

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概要

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• Cationic polyelectrolytes (chitosan, poly-L-lysine (PLL), polyethyleneimine (PEI) and trimethylammonium glycol chitosan iodide (TGCI)) were used to entrap anoxygenic phototrophic bacteria in order to prevent the inhibitory effect of NH^+_4 on hydrogen production. When combined with agar gel, chitosan and PLL demonstrated no obvious repressive effect on hydrogen production by Rhodobacter sphaeroides under light-anaerobic conditions with lactate and glutamate as the carbon and nitrogen sources, respectively. On the other hand, both PEI and TGCI exerted a detrimental effect on hydrogen production under these conditions. Hydrogen production in the presence of NH^+_4 by the bacteria entrapped in the complex gel containing chitosan and agar improved considerably compared to that in the control containing only agar. Evidence shows that chitosan improves the hydrogen production via various effects. Diffusion tests demonstrated that the addition of chitosan increased to some extent the resistance to the diffusion of positively charged NH^+_4, but had no effect on negatively charged lactate. A buffer effect in the complex gels was also revealed.

• 社団法人日本生物工学会の論文
• 1999-11-25

著者

• Miyake J

  Dep. Of Bioengineering Graduate School Of Engineering The Univ. Of Tokyo 7-3-1 Hongo Bunkyo-ku Tokyo

• SUZUKI Tomoo

  Division of Materials Science and Engineering, Graduate School of Engineering, Hokkaido University

• WAKAYAMA TATSUKI

  Tissue Engineering Research Center, National Institute of Advanced Industrial Science and Technology

• MIYAKE Jun

  National Institute for Advanced Interdisciplinary Research, Agency of Industrial Science and Technol

• WAKAYAMA TATSUKI

  National Institute of Bioscience & Human-Technology (NIBH), AIST

• ZHU HEGUANG

  Division of Agricultural Science, The United Graduate School, Tokyo University of Agricultute and Te

• ASADA YASUO

  National Institute of Biosciencd and Human-Technology

• Miyake J

  Fermentation Research Inst. Ibaraki Jpn

• Miyake Jun

  Department Of Bioengineering Graduate School Of Engineering The University Of Tokyo:research Institu

• Miyake Jun

  Department Of Mechanical Science And Bioengineering Graduate School Of Engineering Science Osaka Uni

• Miyake J

  Tissue Engineering Research Center (terc) National Institute Of Advanced Industrial Science And Tech

• Asada Y

  National Institute Of Bioscience And Human-technology Aist Miti

• Zhu Heguang

  Division Of Agricultural Science The United Graduate School Tokyo University Of Agricultute And Tech

• Miyake J

  Tokyo Univ. Agric. & Technol.:rice Aist

• Asada Yasuo

  National Institute Of Bioscience And Human-technology

• Wakayama Tatsuki

  Tissue Engineering Research Center National Institute Of Advanced Industrial Science And Technology

• Miyake Jun

  National Institute For Advanced Interdisciplinary Research Aist

• Suzuki Tomoo

  Division Of Agricultural Science The United Graduate School Tokyo University Of Agricultute And Tech

• Miyake Jun

  National Institute Of Bioscience And Human-technology

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