Actin-based biomechanical features of suspended normal and cancer cells(CELL AND TISSUE ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
The mechanical features of individual cells have been regarded as unique indicators of their states, which could constantly change in accordance with cellular events and diseases. Particularly, cancer progression was characterized by the disruption and/or reorganization of actin filaments causing mechanical changes. Thus, mechanical characterization of cells could become an effective cytotechnological approach for early detection of cancer. To develop mechanical cytotechnology, it would be necessary to clarify the mechanical properties in various cell adhesion states. In this study, we investigated the surface mechanical behavior of cancer and normal cells in the adherent and suspended states using atomic force microscopy. Adherent normal stromal cells showed high surface stiffness due to developed actin cap structures on their apical surface, whereas cancer cells did not have developed filamentous actin structures, and their surface stiffness was low. Upon cell detachment from the substrate, filamentous actin structures of adherent normal stromal cells reorganized to the cortical region and their surface stiffness decreased consequently however, the stiffness of suspended normal cells remained higher than that of cancer cells. These suspended state actin structures were similar, regardless of the cell type. Furthermore, the mechanical responses of the cancer and normal stromal cells to perturbation of the actin cytoskeleton were different, suggesting distinct regulatory mechanisms for actin cytoskeleton in cancer and normal cells in both adherent and suspended states. Therefore, cancer cells possess specific mechanical and actin cytoskeleton features different from normal stromal cells.
著者
-
Kihara Takanori
Department Of Bioengineering Graduate School Of Engineering The University Of Tokyo
-
Shimizu Yuji
Department Of Bioscience And Biotechnology Faculty Of Agriculture Shinshu University
-
Miyake Jun
Department Of Bioengineering Graduate School Of Engineering The University Of Tokyo
-
Miyake Jun
Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University
-
Haghparast Seyed
Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University
-
Yuba Shunsuke
Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
関連論文
- Comparison of efficiency of terminal differentiation of oligodendrocytes from induced pluripotent stem cells versus embryonic stem cells in vitro(CELL AND TISSUE ENGINEERING)
- 2P223 培養細胞へのナノニードル挿入に対するフィブロネクチンナノ薄膜の効果(細胞生物的課題(接着,運動,骨格,伝達,膜),第48回日本生物物理学会年会)
- Correlation of left ventricular dyssynchrony with myocardial stunning using dual single photon emission computed tomography of ^iodine-β-methyl iodophenyl pentadecanoic acid and ^thallium scintigraphy after reperfusion therapy
- Association between brachial-ankle pulse wave velocity and 3-year mortality in community-dwelling older adults
- B-type natriuretic peptide is predictive of hospitalization in community-dwelling elderly without heart diseases
- PJ-637 Clinical Profiles of Hypertrophic Cardiomyopathy with Apical Phenotype : Comparison of Pure-apical Form and Distal-dominant Form(PJ107,Cardiomyopathy/Hypertrophy (Basic, Clinical) (M),Poster Session (Japanese),The 73rd Annual Scientific Meeting of
- Improvement in prognosis of dilated cardiomyopathy in the elderly over the past 20 years
- Onset timing of transient gene expression depends on cell division(CELL AND TISSUE ENGINEERING)
- Interobserver Variation in Diagnosis of Dementia by Brain Perfusion SPECT
- Surface Plasmon Excitation and Emitted Light Properties in Otto/Kretschmann Configuration
- PJ-303 Three-year Dependence in Community-dwelling Elderly can be Predicted by Higher BaPWV Plus TNF-α : Kahoku Study(Atherosclerosis, clinical-09, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- OJ-225 Morning Systolic Blood Pressure at Home on the 2nd Day is the Best Predictor of Prognosis in the Community-dwelling Elderly(Preventive medicine/Epidemiology/Education-2, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- OJ-082 Postural Dysregulation Predicts 5-year Mortality and Impaired Activity of Daily Living in Community-dwelling Elderly : Active 80's Project(Hypertension, clinical-3 (H) OJ14,Oral Presentation (Japanese),The 70th Anniversary Annual Scientific Meeting
- 7 Non-smoking is Important for "Successful Aging of being Active 85" in Community-dwelling Elderly : Ten-year Longitudinal Study(Tobacco Free Symposium: The Present Condition of Tobacco Control in Japan, the Risk of Smoking to Cardio-and Cerebro-Vascular
- Expression and Localization of Galectin-9 in the Human Uterodome
- Intercellular Accumulation of Type V Collagen Fibrils in Accordance with Cell Aggregation
- Reorganization of Type V Collagen Fibrils on Cellular Surface depends on Cell Origin.
- INTERACTIONS OF VASCULAR ENDOTHELIAL CELLS AND SMOOTH MUSCLERELATED CELLS WITH TYPE IV COLLAGEN
- AN-P34 The generation of oligodendrocytes from induced pluripotent stem cells(Section I Animal and Plant Cell Cultures)
- PJ-183 Post-operative Pleural Effusion in Off-pump Surgery Era(Cardiovascular Surgery/CABG 2 (IHD) : PJ31)(Poster Session (Japanese))
- New Plant Growth Promoters, Repraesentins A, B and C, from Lactarius repraesentaneus(Organic Chemistry)
- Ultrasound-mediated Transfection with Liposomal Bubbles Delivers Plasmid DNA Directly into Nucleus
- Effect of composition, morphology and size of nanozeolite on its in vitro cytotoxicity(MEDICAL BIOTECHNOLOGY)
- Malignant Pleural Effusion and Osteoblastic Metastases as the Initial Manifestation of Occult Gastric Cancer : : A Case Report
- Evaluation method for gene transfection by using the period of onset of gene expression and cell division(CELL AND TISSUE ENGINEERING)
- Development of a microfabricated device for low-voltage electropermeabilization of adherent cells(CELL AND TISSUE ENGINEERING)
- Fabrication of in vitro three-dimensional multilayered blood vessel model using human endothelial and smooth muscle cells and high-strength PEG hydrogel(CELL AND TISSUE ENGINEERING)
- Actin-based biomechanical features of suspended normal and cancer cells(CELL AND TISSUE ENGINEERING)
- Maturation timing of stamens and pistils in the dioecious plant Silene latifolia
- Development of a microfabricated device for low-voltage electropermeabilization of adherent cells