Method for Reverse Transfection Using Gold Colloid as a Nano-Scaffold(CELL AND TISSUE ENGINEERING)

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概要

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• DNA microarray of non-viral reverse transfection in cell engineering allows drastic downsizing of large-scale functional screening of genes and siRNAs. However the control of localizability and efficiency of the microarray is still considered as a critical barrier in practical use. One of the major breakthrough to increase the transfection efficiency may be control in the condition of DNA/transfection reagent complex on the microarray surface. In this paper, we showed that negatively charged gold colloid (GC) is successfully used to control the DNA/reagent complex on a glass surface. The conjugation of gold nanoparticles (20nm in diameter) to the pEGFP-N1/Jet-PEI complex resulted in a more than 2.5-fold increase in the intensity of fluorescence of enhanced green fluorescent protein (EGFP) (based on the efficiency of transfection) from human mesenchymal stem cells (hMSCs), as compared to the control without GC. Our method for reverse transfection should be useful not only for cell array-based analyses but also as a novel gene-delivery method for gene therapy in regenerative medicine.

• 社団法人日本生物工学会の論文
• 2007-01-25

著者

• Miyake Jun

  Research Institute of Cell Engineering, National Institute of Advanced Industrial Science and Techno

• Fujita Satoshi

  Research Institute For Cell Engineering (rice) National Institute Of Advanced Industrial Science And

• Fujita Satoshi

  Department Of Bioengineering School Of Engineering The University Of Tokyo:research Institute For Ce

• YAMADA Shigeru

  Research Center Hospital for Charged Particle Therapy, National Institute of Radiological Sciences

• Miyake Masato

  Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science an

• Uchimura Eiichiro

  Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science an

• Uebersax Lorenz

  Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science an

• Miyake Masato

  Research Institute For Cell Engineering (rice) National Institute Of Advanced Industrial Science And

• Yamada Shigeru

  Research Institute For Cell Engineering (rice) National Institute Of Advanced Industrial Science And

• Miyake Jun

  Department Of Bioengineering School Of Engineering The University Of Tokyo:research Institute For Ce

• Uebersax Lorenz

  Research Institute For Cell Engineering (rice) National Institute Of Advanced Industrial Science And

• Uchimura Eiichiro

  Research Institute For Cell Engineering (rice) National Institute Of Advanced Industrial Science And

• Fujita Satoshi

  Department Of Bioengineering School Of Engineering The University Of Tokyo:research Institute For Ce

• Miyake Masato

  Research Institute For Cell Engineering (rice) National Institute Of Advanced Industrial Science And

• Miyake Jun

  Research Institute For Cell Engineering National Institute Of Advanced Industrial Science And Techno

• Yamada Shigeru

  Research Center For Charged Particle Therapy National Institute Of Radiological Sciences

• Yamada Shigeru

  Research Center For Advanced Science And Technology The University Of Tokyo

• Miyake Jun

  Research Institute For Cell Engineering (rice) National Institute Of Advanced Industrial Science And

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