Steady-State Near-infrared Detection of Singlet Molecular Oxygen : A Stern-Volmer Quenching Experiment with Luminol, Superoxide Dismutase, and Cypridina Luciferin Analogues(Organic Chemistry)
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概要
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A sensitive near-infrared detection system with improvements was used to study the quenching of the steady-state luminescence of singlet molecular oxygen at 1270 nm. Stern-Volmer plots which were linear up to 80% quenching by luminol of the ^1O_2 generated by rose bengal yielded rate constants of 9.30×10^8 and 1.40v10^9M^<-1> sec^<-1> for the quenching of ^1O_2 in pH 7.1 and pH 10.1 buffer solutions (25 mM). Quenching rate constants of ^1O_2 by superoxide dismutase and Cypridina luciferin analogues, 2-methyl-6-phenyl- and 2-methyl-6-[p-[2-[sodium 3-carboxylato-4-(6-hydroxy-3-xanthenon-9-yl)-phenylthioureylene]ethyleneoxy]phenyl]-3,7-dihydroimidazo[1,2-α]pyrazin-3-one (CLA and FCLA) were measured similarly as 2.73v10^9, 6.30×10^8 and 8.00×10^8M^<-1>sec^<-1> in the pH 7.1 buffer solutions, respectively.
- 社団法人日本農芸化学会の論文
- 1990-11-23
著者
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MASHIKO Shinro
Communication Research Laboratory
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Mashiko Shinro
Kansai Advanced Research Center, National Institute of Information and Communication Technology
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Suzuki Nobutaka
Department Of Food Science National University Of Fisheries
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Suzuki Nobutaka
Faculty Of Computer Science And System Engineering Okayama Prefectural University
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Inaba Humio
Biophoton Project Research Development Corporation Of Japan (jrdc):research Institute Of Electrical
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Toya Yoshiaki
Aichi University Of Education
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Mashiko Shinro
Kansai Advanced Research Center National Institute Of Information And Communication Technology
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Mashiko Shinro
Communications Research Laboratory Ministry Of Posts And Telecommunications Kansai Advanced Research
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Suzuki N
Shizuoka Univ. Shizuoka Jpn
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INABA Humio
Yamagata Advanced Technology Research and Development Center, Tohoku Institute of Technology
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Inaba Humio
Yamagata Advanced Technology Research And Development Center Tohoku Institute Of Technology
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MIZUMOTO Iwao
Toyama National Institute of Maritime Technology
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NOMOTO Tateo
Department of Chemistry, Mie University
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NOMOTO Tateo
Faculty of Education, Mie University
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SUZUKI Nobutaka
Biophoton Project, Research Development Corporation of Japan (JRDC)
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MIZUMOTO Iwao
Research Institute of Electrical Communication, Tohoku University
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TOYA Yoshiaki
Department of Chemistry, Aichi University of Education
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Suzuki Nobutaka
Biophoton Project Research Development Corporation Of Japan(jrdc)
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Nomoto Tateo
Department Of Chemistry Mie University
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