Glycolytic Inhibitor Failed to Inhibit Superoxide Generation in Mouse Macrophages : An Investigation Using a Chemiluminescence Probe Specific for Superoxide Anion(Biological Chemistry)
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概要
- 論文の詳細を見る
Glucose is widely known to be required during superoxide (O_2^-) generation in phagocytic cells. However, when an O_2^--specific chemiluminescence probe with the Cypridina luciferin analog 2-methyl-6-(p-methoxyphenyl)-3,7-dihydro-imidazo[1,2-a]pyrazin-3-one (MCLA) was used, about 60% of the chemiluminescence remained in stimulated macrophages in the presence of the glycolytic inhibitor 2-de-oxyglucose. O_2^--nonspecific luminol-dependent chemiluminescence disappeared when the same drug was added. These results clearly demonstrate that the generation of O_2^- by macrophages is not completely glucose-dependent, and strongly suggest that macrophages have both glucose-independent NADPH-supplying pathway(s) and glucose dependent pathway(s) which generate reactive oxygen species other than O_2^-.
- 社団法人日本農芸化学会の論文
- 1989-04-23
著者
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Kimura Shuichi
Faculty Of Agriculture Tohoku University
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Hosono Akiyoshi
Department Of Animal Husbandry Faculty Of Agriculture Shinsyu University
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Kimura S
Protein Engineering Research Inst. Osaka Jpn
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Inaba Humio
Biophoton Project Research Development Corporation Of Japan (jrdc):research Institute Of Electrical
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Hosono Akira
Faculty Of Agriculture Tohoku University
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Mashiko Shinroh
Biophoton Project Research Development Corporation Of Japan(jrdc):(present Office)communications Res
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INABA Humio
Yamagata Advanced Technology Research and Development Center, Tohoku Institute of Technology
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HISHINUMA Kohya
Biophoton Project, Research Development Corporation of Japan
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Inaba Humio
Yamagata Advanced Technology Research And Development Center Tohoku Institute Of Technology
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Hishinuma Kiyoshi
Biophoton Project Research Development Corporation Of Japan
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Mashiko Shinroh
Biophoton Project Research Development Corporation Of Japan
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