Antigenic Reactive Regions of S-Carboxymethylated β-Lactoglobulin(Food & Nutrition)

概要

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Rabbit antibody to reduced carboxymethylated bovine β-lactoglobulin (SCM-β-Lg) was prepared and characterized in order to study the antigenic property of bovine β-lactoglobulin (β-Lg) which is a potential cause of an allergy to cow's milk. On immunodiffusion analysis, a clear, sigle precipitin are was produced between SCM-β-Lg and its antiserum, while a barely discernible line was observed between β-Lg and anti SCM-β-Lg antiserum. Analysis of the quantitative precipitation of SCM-β-Lg with β-Lg-absorbed antiserum to SCM-β-Lg indicated that there were at least four antigenic sites on SCM-β-Lg.In a quantitative precipitin inhibition test and a two-step inhibition test involving an enzymelinked immunosorbent assay, anti SCM-β-Lg antibody reacted with peptides 1〜65, 25〜61, 25〜107, 41〜107, 62〜107, 108〜145, 125〜145 and 146〜162 from β-Lg, whereas peptides located in the N-terminal region, i.e., 1〜7, 8〜24 and 25〜40, and peptid 108〜124 showed little or no measurable affinity for antibody to SCM-β-Lg. These results indicate that SCM-β-Lg has at least four antigenic sites, which are associated with regions 41〜61, 62〜107, 125〜145 and 146〜162 in the primary structure of β-Lg.
社団法人日本農芸化学会の論文
1987-02-23