Spatiotemporal Analysis of the Molecular Interaction between PICK1 and PKC
スポンサーリンク
概要
- 論文の詳細を見る
PICK1 is a protein which was initially identified as a protein kinase Cα (αPKC) binding protein using the yeast two-hybrid system. In addition to αPKC, the PICK1 complex binds to and regulates various transmembrane proteins including receptors and transporters. However, it has not been clarified when and where PICK1 binds to αPKC. We examined the spatiotemporal interaction of PICK1 and PKC using live imaging techniques and showed that the activated αPKC binds to PICK1 and transports it to the plasma membrane. Although the membrane translocation of PICK1 requires the activation of αPKC, PICK1 is retained on the membrane even after PKC moves back to the cytosol. These results suggest that the interaction between αPKC and PICK1 is transient and may not be necessary for the regulation of receptors/transporters by PICK1 or by αPKC on the membrane.
- 日本組織細胞化学会の論文
著者
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SAKAI Norio
Department of Molecular and Pharmacological Neuroscience, Graduate School of Biomedical Sciences, Hi
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斎藤 尚亮
神戸大学・バイオシグナル研究センター
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斎藤 尚亮
神戸大、バイオシグナル研究センター
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斉藤 尚亮
神大バイオシグナル
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斎藤 尚亮
神大バイオシグナル
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Sakai Norio
Department Of Molecular And Pharmacological Neuroscience Graduate School Of Biomedical Sciences Hiro
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Saito Naoaki
Department Of Molecular And Pharmacological Neuroscience Graduate School Of Biomedical Sciences Hiro
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Saito Naoaki
Lab. Of Mol. Pharmacol. Biosignal Res. Ctr. Kobe University:grad Sch. Of Sci. & Tech. Kobe Unive
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Saito Naoaki
Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Japan
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SHIRAI Yasuhito
Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University
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斎藤 尚亮
神戸大学バイオシグナル研究センタｰ分子薬理分野
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Saito N
Department Of Molecular And Pharmacological Neuroscience Graduate School Of Biomedical Sciences Hiro
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Masukawa Kenji
Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University
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Ohmori Shiho
Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University
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Masukawa Kenji
Laboratory Of Molecular Pharmacology Biosignal Research Center Kobe University
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Ohmori Shiho
Laboratory Of Molecular Pharmacology Biosignal Research Center Kobe University
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Saito Naoaki
Laboratory Of Molecular Pharmacology Biosignal Research Center Kobe Univ.
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Sakai Norio
Department Of Developmental Medicine (pediatrics) D-5 Osaka University Graduate School Of Medicine.
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Sakai Norio
Department Of Biopharmaceutics And Drug Rational Research Center School Of Pharmacy Tokyo University
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Shirai Yasuhito
Laboratory Of Molecular Pharmacology Biosignal Research Center Kobe Univ.
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Shirai Yasuhito
Laboratory Of Biochemistly Faculty Of Agriculture Kobe University
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Sakai Norio
Department Of Molecular And Pharmacological Neuroscience Graduate School Of Biomedical Sciences Hiro
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