オープンウェル型マイクロチップを用いる酵素免疫測定法の開発

概要

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An enzyme-linked immunosorbent assay (ELISA) on the surface of a glass open-well microchip (4mmφ, 10μm Depth) was developed. The analytical principle of this method for the determination of immunoglobulin A (IgA) was the same as the conventional sandwich-type ELISA. Firstly, a primary antibody (anti-IgA) was adsorbed on the surface of an open-well microchip, and then an antigen (IgA) and secondary antibody (anti-IgA HRP labeled) were reacted with the adsorbed antibody. The HRP remained on the surface of the open-well microchip, which was determined by using Amplex^[○!R] Red System with an incident-light fluorescence microscope after the reacyion for 90 seconds. The calibration curve of the IgA standard solution was linear in the range up to 5 ng/ml on 10μl of the substrate volume. The proposed method with the openwell microchip shortened the reaction time compared to the conventional ELISA with a 96-well plate.
社団法人日本分析化学会の論文
2006-05-05