アゾベンゼン修飾キラル固定相を用いるマイクロ液体クロマトグラフィーにおける光学異性体の光照射による溶出順序制御

概要

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In micro liquid chromatography, a method for the reversible control of the elution order of enantiomers by light irradiation was developed. Two photo-responsive chiral stationary phases (CSP(1) and CSP(2)) with an azoberizene moiety were prepared using D- and L-valine, respectively. The prepared stationary phases were continuously packed into a UV-permeable polymercoated fused-silica capillary tubing (100μm, i.d.) with 20 cm of length, respectively, and used as a micro column. When 3,5-dinitrobenzoyl-D,L-leucine isopropyl ester and 3,5-dinitrobenzoyl-D,L- alanine methyl ester were used as test samples, the samples were eluted in the order D-form and L-form, after UV light was irradiated to only the part of CSP(1) in the column. Continuously, when UV light was irradiated to the part of CSP(2) after visible light was irradiated to the CSP(1), the elution order became L-form and D-form. It could be seen that a reversible change in the retention order was caused by the reduction of the enantio-selectivity of the stationary phase by UV light irradiation.
社団法人日本分析化学会の論文
2003-10-05