A New Method to Quantitate Lipid Contents in Human Platelets by Thin Layer Chromatography with Flame lonization Detection
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概要
- 論文の詳細を見る
Lipid contents in human platelets from 21 healthy adults were analysed using thin-layer chromatography (TLC) in combination with a flame ionization detector (FID). Samples of 0.3μl extracted from 10^8 platelets were chromatographed on quartz rods coated with silica gel (Chromarods), and calibrated with an FID using free cholesterol and phosphatidylcholine as standards. The weight percentage of neutral lipids in human platelets obtained in this study was 14.6% which consisted mainly of free cholesterol, while that of phosphatidylethanolamine was 24.9%, phosphatidylserine plus phosphatidylinositol 6.8%, phosphatidylcholine 35.2% and sphingomyelin 18.6%. Free cholesterol in 10^8 platelets was estimated as 7μg and phospholipids as 46μg from standard calibrations. The minimum amount detectable by this TLC-FID method was 0.05μg of standard cholesterol acetate and 0.1μg of lipids from 10^8 human platelets. The reproducibility was quite satisfactory and the procedure could be performed quickly and simply.
- 東海大学の論文
著者
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Arimori Shigeru
The Fourth Department Of Internal Medicine Tokai University
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Hiramatsu Kazuko
Department Of Internal Medicine School Of Medicine Tokai University
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Nozaki Hiroyuki
Department Of Internal Medicine School Of Medicine Tokai University
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NOZAKI Hiroyuki
The Fourth Department of Internal Medicine, School of Medicine, Tokai University
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HIRAMATSU Kazuko
The Fourth Department of Internal Medicine, School of Medicine, Tokai University
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Nozaki Hiroyuki
The Fourth Department Of Internal Medicine School Of Medicine Tokai University
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Hiramatsu Kazuko
The Fourth Department Of Internal Medicine School Of Medicine Tokai University
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Arimori Shigeru
The Fourth Department Of Internal Medicine School Of Medicine Tokai University
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HIRAMATSU Kazuko
Department of Internal Medicine, School of Medicine, Tokai University
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