Effect of Pantethine on ADP-Induced Aggregation, Microviscosity and Ultramicrostructure of Human Platelets

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Pantethine is a constituent of Coenzyme A. The present study was undertaken to investigate the effect of pantethine on the function and morphology of human platelets in vitro. Platelet suspensions from 43 subjects including healthy subjects and patients with various disorders were incubated with pantethine in vitro and the patterns of platelet aggregation following addition of ADP after 5 minutes of incubation were determined with a Sienco Dual Sample Aggregation Meter. The data showed decreased ADP-induced aggregation in 17 cases (40%), a considerable proportion of which had hyperlipidemia. Four patients (9%), none of whom were hyperlipidemic, showed enhanced ADP-induced platelet aggregation. To clarify this discrepancy, the timecourse of changes in ADP-induced platelet aggregation was investigated in a patient with nephrotic syndrome associated with pronounced hyperlipidemia. It has been inferred from the results that pantethine may enhance the function of the platelet membrane in the initial course and, subsequently, increase the activity and preserve the ultramicrostructures of intracellular organellae. For instance, pantethine caused the microviscosity of diabetic platelets, which was measured with a Elscint microviscosimeter Model MV-la using DPH as a probe, to become approximately the same as that of healthy platelets. Electron microscopic observations of the platelets provided morphologic evidence in support of these effects of pantethine on platelet function. The spontaneous course of platelet degeneration was delayed significantly in the presence of pantethine which was demonstrated on mitochondria, alpha-granules, dense bodies, glycogen granules, tubular systems and the cell matrix, as well as the platelet membrane.
東海大学の論文

Effect of Pantethine on ADP-Induced Aggregation, Microviscosity and Ultramicrostructure of Human Platelets

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