Fundamental Studies on Human T Lymphocyte Subsets

概要

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Human T lymphocytes bearing receptors for IgG antibodies (T γ cells) and those possessing receptors for IgM antibodies (T μ cells) were studied using the rosette technique with bovine erythrocytes coated with either I gG (BEA (IgG)) or IgM (BEA (I gM)). The T lymphocytes were purified as spontaneous rosette forming cells (RFC) with sheep red cells. A forcible adherence of BEA (I gG) to lymphocytes with centrifugation was required for the detection of T γ cells. The highest proportion of BEA (I gG)-RFC was obtained by incubating at 37℃ for 60 min. BEA (I gM)-RFC appeared after an 8 hr incubation and reached a plateau during 12 to 20 hr of incubation. The number of BEA (I gM) required to form a rosette with each lymphocyte increased in accordance with the incubation period. Centrifugation was not required to detect T μ cells. The BEA (I gM)-RFC was inhibited remarkably by addition of I gM molecules to the culture medium. However, the addition of I gG molecules to the medium failed to inhibit BEA (I gM)-RFC of T lymphocytes. These results appear to indicate that both affinity of antibodies to their surface receptors and the kinetics of their receptors are different in T γ and T μ cells.
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