SEMI-QUANTITATIVE IMMUNOHISTOCHEMICAL ANALYSIS OF MALE RAT-SPECIFIC α_2u-GLOBULIN ACCUMULATION FOR CHEMICAL TOXICITY EVALUATION
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概要
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We purified male rat urinary α_2u-globulin, prepared the antibody in rabbits, and improved an immunohistochemical detection method using this antibody for male rat-specific α_2u-globulin accumulation appearing as hyaline droplets in the kidneys. Our prepared antibody reacted specifically with α_2u-globulin in both immunohistochemical and Western blotting analyses, furthermore, and the graded immuno-reactivities on the slide were well associated with computational image analyzing results. Using this method, we retrospectively analyzed the renal sections from the toxicity studies of 12 nephrotoxic chemicals, which had already been conducted under the Japanese Existing Chemicals Survey Program. We demonstrated that the hyaline droplets induced by treatment with 10 chemicals (1, 4-dibromobenzene, dicyclopentadiene, 3, 4-dimethylaniline, 1, 4-dicyanobenzene, tetrahydrothiophene-1, 1-dioxide, 1, 3-dicyanobenzene, acenaphthene, 3, 4-dichloro-1-butene, 3a, 4, 7, 7a-tetrahydro-1H-indene and 3, 5, 5-trimethylhexan-1-ol) were directly associated with α_2u-globulin accumulation. This immunohis-tochemical method is convenient for applying, even retrospectively, paraffin sections from general toxicity studies and could be useful for qualifying male rat-specific hyaline droplets consisting of α_2u-globulin and renal risk in humans.
- 日本トキシコロジー学会の論文
- 2006-02-24
著者
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Hirose Akihiko
National Institute Of Health Sciences
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Hirose Akihiko
National Inst. Of Health Sciences
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KAMATA Eiichi
National Institute of Health Sciences
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Hasegawa Ryuichi
ボゾリサーチセンター
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Hasegawa Ryuichi
National Institute Of Health Sciences
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Hasegawa Ryuichi
Division Of Toxicology National Institute Of Health Sciences
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Hirose Akihiko
Division Of Risk Assessment National Institute Of Health Sciences
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Kamata E
National Institute Of Health Sciences
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Hirose Akihiko
Division Of Toxicology National Institute Of Health Sciences
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Ema Makoto
National Institute of Health Sciences, Osaka Branch
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Kamata Eiichi
Division Of Risk Assessment Biological Safety Research Center National Institute Of Health Sciences
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Hasegawa Ryuich
Division Of Toxicology National Institute Of Health Sciences
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HAMAMURA Masao
Panapharm Laboratories Co., Ltd.
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KATOKU Koshiro
Panapharm Laboratories Co., Ltd.
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KUWASAKI Emiko
Panapharm Laboratories Co., Ltd.
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OSHIKATA Takafumi
Panapharm Laboratories Co., Ltd.
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NAKAHARA Yutaka
Panapharm Laboratories Co., Ltd.
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Hasegawa Ryuichi
Division Of Medical Safety Science
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Hasegawa Ryuichi
Department Of Intensive Care Medicine Tohoku University Hospital
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Ema M
National Institute Of Health Sciences
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Ema Makoto
National Institute Of Health Sciences Osaka Branch
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Ema Makoto
National Institute Of Hygienic Sciences Osaka Branch
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Hamamura Masao
Panapharm Laboratories Co. Ltd.
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Kuwasaki Emiko
Panapharm Laboratories Co. Ltd.
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Kamata Eiichi
National Institute Of Hygienic Sciences
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Hirose Akihiko
National Inst. Of Health Sciences (nihs)
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Nakahara Yutaka
Panapharm Laboratories Co. Ltd.
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Kamata Eiichi
National Inst. Of Health Sciences (nihs)
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Oshikata Takafumi
Panapharm Laboratories Co. Ltd.
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Hamamura Masao
Kumamoto Laboratory Mitsubishi Chemical Safety Institute Ltd.
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KATOKU Koshirou
Kumamoto laboratory, Mitsubishi Chemical Safety Institute Ltd.
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OSHIKATA Takafumi
Kumamoto laboratory, Mitsubishi Chemical Safety Institute Ltd.
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Oshikata Takafumi
Kumamoto Laboratory Mitsubishi Chemical Safety Institute Ltd.
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Katoku Koshiro
Kumamoto Laboratory Mitsubishi Chemical Safety Institute Ltd.
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Ando Hiroshi
Department Of Environmental Health And Toxicology Tokyo Metropolitan Institute Of Public Health
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Ema Makoto
National Institute Of Health Sciences
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