Repression of Fibroblast Migration into the Collagen Gel Incubated with Glucose Is Due to Glycation-mediated Cross-linking of Collagen
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概要
- 論文の詳細を見る
The chemical properties of reconstituted collagen fibrils were changed by prolonged incubation with a high concentration of glucose. The amounts of lysine and arginine residues in the collagen gels decreased after incubation with 0.56M glucose for two weeks or more, suggesting that glycation of the residues had occurred. CNBr treatment of collagen incubated with glucose yielded polypeptides migrating as bands with lower mobilities on SDS-PAGE than expected for those obtained from collagen not incubated with glucose. Extraction with 0.5M acetic acid in the presence or absence of pepsin left a greater amount of insoluble materials in the case of glucose-incubated collagen. These results suggest that the glycation of polypeptides eventually led to the formation of covalent cross-links. Interactions between fibroblasts and the collagen gel were examined to see whether the biological activity of collagen gel was changed by the glycation. Cell attachment, spreading, and proliferation on the glycated collagen gel were essentially unaffected, but cell migration from the surface into the collagen gel was markedly inhibited. The cross-linking of collagen gel with NaIO_4 also inhibited the cell migration into the gel, but did not affect proliferation. These observations suggest that extensive glycation leads to the cross-linking of collagen and that cross-linking of collagen, regardless of its chemical nature, inhibits cell migration.
- 日本結合組織学会の論文
- 2000-09-25
著者
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林 利彦
東京大学 大学院 総合文化研究科 生命環境系
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林 利彦
東大・総合文化
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HAYASHI Toshihiko
Department of Life Sciences-Chemistry, Graduate School of Arts and Sciences, The University of Tokyo
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NISHIYAMA Toshio
Shiseido Life Science Research Center
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AKUTSU Nobuko
Shiseido Life Science Research Center
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Horii Izumi
Shiseido Skin Res. Lab.
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Akutsu N
Shiseido Life Sci. Res. Center
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Horii Izumi
Shiseido Life Science Research Center
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Hayashi Toshihiko
Department Of Biology Faculty Of Science Shizuoka University
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Hayashi Toshihiko
Department Of Life Sciences College Of Arts And Sciences The University Of Tokyo
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Hayashi Toshihiko
Department Of Life Sciences Graduate School Of Arts And Scienses The University Of Tokyo
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