Studies on the Hydroxylation of Phenylalanine by 6,7-Dimethyl-5,6,7,8-tetrahydropteridine
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概要
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The hydroxylation of phenylalanine by 6,7-dimethyl-5,6,7,8-tetrahydropteridine (DMPH_4) was investigated. When phenylalanine was treated with DMPH_4 in citrate buffer (pH 6.0), p-tyrosine, m-tyrosine and o-tyrosine were identified as hydroxylated products. The hydroxylation was pH-dependent, and the maximum rate was found at around pH 6. Replacement of air with nitrogen gas and the addition of hydroxyl radical scavengers or catalase prevented the hydroxylation. In contrast, ferrous ions significantly accelerated the hydroxylation as compared with other transition metal ions. In an aqueous solution of DMPH_4 under aerobic conditions, the electron spin resonance (ESR) spectra of the hydroxyl radical spin adducts with spin traps such as α-phenyl N-tert-butyl nitrone (PBN) and α-4-pyridyl 1-oxide N-tert-butyl nitrone (4-POBN) were observed. The results indicate that the hydroxylating effect of DMPH_4 is caused by hydroxyl radicals formed during the autooxidation of DMPH_4.
- 公益社団法人日本薬学会の論文
- 1984-02-25
著者
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石光 進
Kyoto Phramaceutical Univeristy
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小原 晃
Kyoto College of Pharmacy
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小原 晃
Laboratory Of Biochemistry Kyoto Pharmaceutical University
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藤本 貞毅
Kyoto Pharmaceutical University
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藤本 貞毅
Laboratory Of Biochemistry Kyoto Pharmaceutical University
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