Purification and Characterization of Zinc-Dependent Acid Phosphatase from Bovine Brain
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概要
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Zn^<2+>-Dependent acid phosphatase (Zn^<2+>-APase) was purified to homogeneity from bovine brain. The apparent molecular weight of the enzyme was estimated to be about 62000 by gel filtration and 31000 by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The enzyme exhibited an isoelectric point of approximately 4.8. The enzyme required Zn^<2+> ions for catalytic activity, but other cations had little or no effect. The maximum enzyme activity was obtained in the presence of about 5mM of Zn^<2+> at pH 5.5 in 50mM 2-(N-morpholino)ethanesulfonic acid-NaOH buffer. The enzyme significantly catalyzed the hydrolysis of p-nitrophenyl phosphate, phenyl phosphate, and phosphotyrosine. The enzyme was also active for myo-inositol-2-monophosphate and adenosine 2'-monophosphate of the other common phosphate esters tested, though significantly less active than for p-nitrophenyl phosphate. The optimum activity pH of the enzyme was around 5.5 with p-nitrophenly phosphate and myo-inositol-2-monophosphate. The enzyme was resistant to fluoride ions.Two types of Zn^<2+>-APases, a high molecular weight (molecular weight, Mr., about 110000) and a low molecular weight (Mr., about 62000) type, were found to exist in various tissues of rat. Brain, lung, spleen, stomach, heart, skeletal muscle, and erythrocytes contained only the lower molecular weight type. On the other hand, liver and kidney contained mainly the higher molecular weight type, and the small intestine contained significant quantities of the both types.
- 社団法人日本薬学会の論文
- 1993-08-15
著者
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小原 晃
Kyoto College of Pharmacy
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小原 晃
Laboratory Of Biochemistry Kyoto Pharmaceutical University
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藤本 貞毅
Kyoto Pharmaceutical University
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藤本 貞毅
Laboratory Of Biochemistry Kyoto Pharmaceutical University
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後藤 博輝
Kyoto Pharmaceutical University
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大林 武
Kyoto Pharmaceutical University
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八崎 裕明
Kyoto Pharmaceutical University
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Ohara A
Kyoto Pharmaceutical Univ. Kyoto Jpn
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Chara A
Laboratory Of Biochemistry Kyoto Pharmaceutical University
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