On the Mechanism of Inactivation of Papain by Hydroxylamine
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概要
- 論文の詳細を見る
The mechanism of inactivation of papain (EC 3.4.4.10) by hydroxylamine was investigated. The inactivation was pH-dependent and the rate was rapid at alkaline pH. Nitrogen gas bubbling through the reaction mixture or addition of radical scavengers to the reaction mixture largely prevented the inactivation. The inactivation was accompanied by a decrease of the essential sulfhydryl group of papain. Treatment of inactivated papain with 2-mercaptoethanol led to partial reactivation. The inactivated papain was judged not to be dimeric on the basis of molecular weight determination.Based on these results, it is likely that hydroxylamine inactivates papain indirectly by reacting with O_2 to produce some reactive species which modifies the essential sulfhydryl group of the enzyme.
- 社団法人日本薬学会の論文
- 1990-02-25
著者
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石光 進
Kyoto Phramaceutical Univeristy
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金沢 治男
Kyoto College of Pharmacy
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小原 晃
Kyoto College of Pharmacy
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小原 晃
Laboratory Of Biochemistry Kyoto Pharmaceutical University
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藤本 貞毅
Kyoto Pharmaceutical University
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藤本 貞毅
Laboratory Of Biochemistry Kyoto Pharmaceutical University
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Ohara A
Kyoto Pharmaceutical Univ. Kyoto Jpn
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Chara A
Laboratory Of Biochemistry Kyoto Pharmaceutical University
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金沢 治男
Kyoto Pharmaceutical University
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