Behavior of Cadmium in Rabbit Blood

概要

論文の詳細を見る
Progressive distribution of ^<115m>Cd in the blood and the main ^<115m>Cd-binding protein in the red cells were investigated after a single intraperitoneal administration of ^<115m>Cd-Cl_2 to rabbit. ^<115m>Cd in blood decreased drastically on the 1st day after injection and increased sharply again on the 2nd day, then gradually decreased showing the regression equation of Y=-0.019x+3.507. The half life of ^<115m>Cd in the blood was calculated to be 158.4 days. On the 14th day after injection of ^<115m>CdCl_2 solution, 99.1% of the radioactivity in the whole blood was in the red cells, over 90% of the radioactivity in the red cells existing in the supernatant of stroma-free hemolysate. From the results of gel-filtration, DEAE Sephadex column chromatography and gel-electrophoresis, it was elucidated that about 70% of the radioactivity of the stroma-free hemolysate was bound to thionein. But the ratio of the two components in metallothionein was different between the red cells and the liver. It was also made clear that incorporation of metallothionein into red cells through cell membrane never occurred by incubating red cells with ^<115m>Cd-thionein. Considering the observations mentioned above, it was presumed that the metallothionein in the red cells may have originated from some other organ than the liver.
公益社団法人日本薬学会の論文
1979-04-25