Radioimmunoassayによる2,5-Dimethoxy-4-methylamphetamineの定量

概要

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A method of radioimmunoassay of 2,5-dimethoxy-4-methylamphetamine (DOM), a hallucinogen, was studied. In order to use for immunization, DOM-glutaraldehydehuman serum albumin conjugate was prepared from DOM, glutaraldehyde, and human serum albumin, followed by purification by dialysis. Anti-DOM antiserum was obtained by repeated injection of the prepared antigen to guinea pig. The method of radioimmunoassay established after some experiments was as follows : To an aliquot of the test serum or sample solution, a definite amount of DOM labeled with ^3H and anti-DOM antiserum were added, then the globulin fraction was isolated by salting out as a precipitate, which contains the bound DOM come from the test serum or sample solution, and labeled DOM. Radioactivity of the precipitate was measured, and the amount of DOM in the test serum or sample solution was determined from a calibration curve, which was prepared with various known amounts of standard DOM. The sensitivity of this radioimmunoassay was 1-100 ng/ml, and the specificity to DOM was so sufficient that several pharmaceuticals such as ephedrine, dopamine and epinephrine showed no disturbance to this method.
公益社団法人日本薬学会の論文
1974-10-25