油脂変質に伴い生成するカルボニル化合物のラット初代培養肝細胞に対する毒性について

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Toxicological studies of carbonyl compounds derived from oxidized lipids on primary cultured rat hepatocytes were investigated. As carbonyl compounds, saturated aldehyde, α, β-unsaturated aldehyde, glyoxals and malondialdehyde were used. Hepatocytes isolated from male Wistar rats by the collagenase perfusion method were cultured in 35 mm dishes in an atmosphere of 5% CO_2-95% air at 37℃. Rat hepatocytes in culture for 24 h were incubated in Williams'E medium containing carbonyl compounds. Hepatotoxicity was estimated as the leakage of hepatic enzymes such as lactate dehydrogenase (LDH) and glutamic-oxalacetic transaminase (GOT) from the cells to medium. LDH and GOT activities were determined by an automatical analyzer. The order of hepatotoxic potency was as follows : glyoxals>α, β-unsaturated aldehydes>saturated aldehydes. Malondialdehyde had not hepatotoxicity. Hepatotoxicity of α, β-unsaturated aldehyde was inhibited by the addition of ascorbic acid and glutathione, whereas that of autoxidized methyl linoleate (peroxide value=2538 meq/kg and carbonyl value=872 mmol/kg) was inhibited by the addition of not only 3-tert-butyl-4-hydroxyanisole (BHA) and N, N'-diphenyl-p-phenylenediamine such as antioxidant, but also ascorbic acid and glutathione. From the above results, it is suggested that both hydroperoxides and α, β-unsaturated aldehydes contribute to the hepatotoxicity of autoxidized methyl linoleates.
公益社団法人日本薬学会の論文
1995-02-28

油脂変質に伴い生成するカルボニル化合物のラット初代培養肝細胞に対する毒性について

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