チオバルビツール酸反応性物質precursorとしての2-alkenal類 : 活性酸素並びに過酸化物による2-alkenal類の酸化分解

概要

論文の詳細を見る
For the evaluation of 2-alkenal as a precursor of thiobarbituric acid reactive substance (TBA-RS), 2-alkenal (C_3-C_9 and 2,4-hexadienal) was incubated with tert-butyl hydroperoxide (t-BuOOH) at 40℃ for 2 hr, Fenton reagent (H_2O_2/Fe^<2+>) or hypoxanthine-xanthine oxidase (Hx-XOD) in citrate buffer at 37℃ for 1 hr. A reaction mixture was made to react with thiobarbituric acid (TBA) in 70% acetic acid at 100℃ for 30 min. The amount of red pigment formed from the 2-alkenal greatly increased by addition of t-BuOOH. These results indicate that the red pigment was formed from 2-alkenal by the addition of t-BuOOH during the incubation, but that was formed in the TBA reaction process. Then, the usage of TBA reagent containing Sn^<2+> (5 μmol), which prevents t-BuOOH oxidation in the TBA reaction, 14.6 and 12.5 mol% of TBA-RS were formed from 2-hexenal and 2,4-hexadienal by heating with t-BuOOH at 100℃ for 60 min, respectively. In Fenton reagent (H_2O_2/Fe^<2+>), the amount of TBA-RS from 2,4-hexadienal decreased to 28% during the reaction at 100℃ for 60 min. In Hx-XOD, TBA-RS from 2-alkenal (C_3,C_4 and C_6) and 2,4-hexadienal increased with superoxide oxidation. From the above results, it is suggested that 2-alkenal was decomposed with t-BuOOH oxidation and Hx-XOD system, and thiobarbituric acid reactive substances were formed.
社団法人日本薬学会の論文
1991-06-30