Imidazole並びにthiazole誘導体-Cu(II)キレートの活性酸素種に対する消去効果

概要

論文の詳細を見る
Scavenged effects of Cu (II) chelates of imidazole and thiazole derivatives on superoxide (SO), H_2O_2 and OH radical were studied and compared with that of cimetidine, which is well known as a superoxide scavenger, and with those of superoxide dismutase (SOD), catalase and Cu (ClO_4)_2. Six of imidazole and 6 of thiazole derivatives were cimetidine and famotidine analogues, respectively. Superoxide scavenged effects (IC_<50>) of Cu (II) chelates were in the range of 0.48-5.67μM determined by the nitroblue tetrazolium method in a phenazine methosulfate-NADH system (method A) and in a xanthineoxidase-hypoxanthine system (method B), whereas the IC_<50> values of SOD were 4.5×10^<-2> and 3.4×10^<-2>μM by the methods of A and B, respectively. No catalase activity of Cu (II) chelates were observed. In the studies of the scavenged effect of Cu (II) chelates on hydroxyl radical, IC_<50> values of Cu (II) chelates of N-methyl-N'-[2-(imidazol-4-yl) methylthioethyl]-thiourea (I-1) and its 5-methylimidazole derivative (I-2), which possessed methylthiourea groups, were 13.7 and 8.0μM, respectively, and the Cu chelate of I-2 had the highest scavenged activity against hydroxyl radical generated from Fenton reaction.
1993-02-28