水溶性ビタミン類及びSH化合物による抗変異原性効果

概要

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The relationship between the inhibitory effects of water soluble vitamins and SH compounds on the formation of O^6-methyldeoxyguanosine (MedGuo) and on the mutagenicity of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) was investigated. Thiamine (B_1), riboflavin (B_2), pyridoxine (B_6), nicotinamide, ascorbic acid (AsA) and dehydroascorbic acid (DHA) were selected as water soluble vitamins, and cysteine (Cys) and glutathione (GSH) were also selected as SH compounds. In the experiments of the inhibitory effects of the compounds on the formation of O^6-MedGuo, MNNG, 2'-deoxyguanosine and each of the above compounds were incubated at 37℃ for 6 h, and the resulting O^6-MedGuo was derivatized to the fluorescent compound. Then, the fluorophore was determined by HPLC, and the formation ratio (%) of O^6-MedGuo was calculated and compared to that in the reaction without vitamin or SH compound. By the addition of SH compounds (100-folds molar of MNNG), the amounts of the formed O^6-MedGuo was depressed to less than 10%. The inhibitory effects of AsA and DHA on the formation of O^6-MedGuo were greater than those of other vitamins. The desmutagenic effects of AsA and Cys on the mutagenicity of MNNG in Salmonella typhimurium TA 100 were observed, and the mutagenicity was reduced to 10% or less by the addition of equimolar AsA and Cys to MNNG. The desmutagenic effects of B_1,B_2 and B_6 on MNNG were not observed.
公益社団法人日本薬学会の論文
1994-02-28