ラット初代培養肝細胞の脂質過酸化に対する2-hexenalの影響

概要

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Hepatocytes isolated from male Wister rats with or without pre-treatment of phenobarbital (PB), 3-methylcholanthrene (3-MC), β-naphthoflavone (β-NF) and dexamethasone (DMX) by the previous method were cultured in dishes in an atmosphere of 5% CO_2-95% air at 37℃. Rat hepatocytes in culture for 24h were incubated in Williams'E medium containing 2-hexenal and tert-butylhydroperoxide. Cell membrane oxidation was measured by the fluorometric thiobarbituric acid (TBA) method. Cell membrane oxidation induced by 2-hexenal and tert-butylhydroperoxide and inhibited by the addition of some antioxdants, was only observed in whole hepatocytes treated with 2-hexenal, but not in the purified cell membrane under the same conditions. Therefore, it is assumed that 2-hexenal converted to active substances could injure the membrane of hepatocytes. In PB pretreated-rat hepatocytes, increasing TBA-RS formation was observed. Finally, it is concluded that 2-hexenal is activated in hepatocytes by CYP2B, and that the membrane lipid peroxidation is caused by active compounds such as radicals.
公益社団法人日本薬学会の論文
1999-12-31