2'-Deoxyguanosineのメチル化剤処理により生成するO^6-methyldeoxyguanosineのケイ光-高速液体クロマトグラフィーによる定量

概要

論文の詳細を見る
High-performance liquid chromatographic determination of O^6-methylguanine (MeGua) was studied using phenacyl bromide as a precolumn derivatization reagent. After the selective condensation of O^6-MeGua to form fluorescent 9-methoxy-6-phenylimidazo [2,1-b] purine (phenyletheno-O^6-MeGua), separation of that compound from guanine and 7-methylguanine was achieved on an ODS column (150 mm×4 mm i. d.) with acetonitrile-50 mM ammonium dihydrogenphosphate solution containing 5 mM Na 1-octanesulfonate (25 : 75), and the separated etheno-O^6-MeGua derivatives was detected with a fluorescence monitor (319 nm for excitation and 404 nm for emission). O^6-MeGua can be detected as phenyletheno-O^6-MeGua by a fluorometric detector at a level of 40 pg (signal/noise=2). 2'-Deoxyguanosine (dGuo) was reacted with various methylating agents to furnish O^6-methyldeoxyguanosine (MedGuo). Then, O^6-MeGua, which is given from O^6-MedGuo by acid hydrolysis, was determined by the fluorometric derivatization.
公益社団法人日本薬学会の論文
1993-08-31