o-Dinitrobenzene-核酸塩基付加物のSalmonella typhimurium株に対する変異原性並びにプリン塩基付加物の構造について

概要

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o-Dinitrobenzene (DNBz) and each nucleic base were made to react with NaOH in dimethylsulfoxide at room temperature for 3-60 h. All of the synthesized compounds were the 2-nitrophenyl adducts of nucleic bases. The mutagenicity test of the synthesized compounds and of o-DNBz were then carried out against Salmonella typhimurium TA98,TA98NR and TA100 without a mammalian metabolic activation system. o-DNBz has no mutagenic potency against all the strains at a dose up to 100 μg. In the compounds with the adducts of nucleic bases, the mutagenic potencies of the adducts of adenine and guanine in TA98 were 50 revertants/100 μg and 255 revertants/10 μg, respectively and the most potent mutagen in TA100 was an adduct of guanine (382 revertants/100μg). Finally, the structures of the adducts of adenine and guanine could be elucidated as N^1-(2-nitrophenyl)-adenine and N^1-(2-nitrophenyl)-guanine, respectively, by the acid and alkaline degradation and by the isopentyl nitrite treatment of these adducts.
公益社団法人日本薬学会の論文
1992-08-31