アセチルアセトン法-高速液体クロマトグラフィーによる油脂中飽和アルデヒド類の定量

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High-performance liquid chromatographic determination for saturated aliphatic aldehydes, specially formaldehyde, acetaldehyde, 1-propanal, 1-butanal, 1-pentanal and 1-hexanal, were studied using a precolumn derivatization reagent acetylacetone. After the selective condensation of these aliphatic aldehydes to form fluorescent dihydrolutidine derivatives, the separation of these compounds was achieved on a ODS column (150×4 mm i.d.) with a linear gradient system of methanol and water (from 50% methanol to 80% methanol in 30 min). The separated lutidine derivatives were detected with a fluorescence monitor (398 nm for excitation and 488 nm for emmission). Three autoxidized unsaturated fatty acid methyl esters and five autoxidized eddible oils were subsequently assayed for 1-propanal (C_3), 1-butanal (C_4), 1-pentanal (C_5) and 1-hexanal (C_6). The peaks of formaldehyde and acetaldehyde were interfered with unknown peaks derived from oxidized lipids. Their detection limits (S/N=2) were 29 ng and 39 ng for 1-propanal and 1-hexanal, respectively. The other carbonyl compounds such as α, β-unsaturated aldehydes and glyoxal derivatives were not interfered by this method.
1994-12-31