β-ラクタム系抗生物質の分析化学的研究(第2報)高速液体クロマトグラフ法による半合成ペニシリン類PC-904およびPC-462の分析

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Separation and determination of new semisynthetic penicillins, sodium (2S, 5R, 6R)-6-[(R)-2-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-2-phenyl acetamido]-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo [3.2.0] heptane-2-carboxylate (PC-904,apalcillin sodium) and sodium (2S, 5R, 6R)-6-[(R)-2-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-2-(4-hydroxyphenyl) acetamido]-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo [3.2.0] heptane-2-carboxylate (PC-462), was carried out by high performance liquid chromatography (HPLC). PC-904 or PC-462 was separated from its penicilloic and penilloic acids under the following conditions : column, stainless steel (2.1 mm i.d.×1 m) packed with Zipax WAX ; column temperature, 40°; mobile phase, 0.01 M pH 3.4 citrate buffer at a flow rate of 0.7 ml/min, and determined by UV photometry at 254 nm. The analytical results obtained by HPLC were in good agreement with those obtained by the iodometric method previously reported, including some samples with were partially decomposed under drastic conditions.
公益社団法人日本薬学会の論文
1979-07-25