Enzyme Linked Immunosorbent Assay (ELISA) Using Monoclonal Antibody to Detect Methamphetamine in Urine and Hair

概要

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We developed an enzyme linked immunosorbent assay using a monoclonal antibody to determine methamphetamine in biological specimens. The monoclonal antibody against methamphetamine was selected from fifteen isolated. Its cross reactivity against nineteen related compounds was investigated. The monoclonal antibody showed very low cross reactivity against ephedrine (0.1%), methylephedrine (1.5%), methoxyphenamine (0.2%), phentermine (0.4%), norephedrine (<0.1%), N, N-dibenzylethylenediamine (0.5%), p-methoxyamphetamine (0.2%), p-hydroxymethamphetamine (1.3%), p-methoxymethamphetamine (3.3%), methylenedioxyamphetamine (0.9%), labetalol (2.6%) and other related compounds (<1.0%), but not against dimethylamphetamine (150%). The detection limit of methamphetamine assay in urine was 0.2μg/ml at the 95% confidence level and the working range 0.3-30μg/ml. The coefficients of variation of the assay for methamphetamine in urine at 1μg/ml were 5.68% for within-run and 8.26% for between-run. The correlation coefficient between the assay and GC/MS of 48 urine specimens was 0.9934. The assay requires 5μl of specimen in 50μl of total assay volume and takes about 1 h for 96 specimens. The assay was applied to hair analysis to monitor methamphetamine abuse history.
社団法人日本薬学会の論文
1989-10-31