デプレニールとメタンフェタミンの尿中代謝物の比較に関する研究(第3報)キラール試薬GITCを用いたHPLCによるマウス尿中代謝物の光学異性体分離分析

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Enantiomeric compositions of methamphetamine (MA) and its major metabolite, amphetamine (AP), in the urine of mice administered with deprenyl (DPN) and MA were analysed by high-performance liquid chromatography using the tetra-O-acetyl-β-D-glucopyranosyl isothiocyanate (GITC) derivatization method for the discrimination between the use of l-DPN and MA enantiomers. l-DPN, d-MA, l-MA and dl-MA were intraperitoneally administered to mice at 1,5 and 10 mg/kg. Urine samples were collected at 0-12,12-24,24-48 and 48-72 h after administration, Urine added with N-methylphenethylamine (internal standard) was applied to BOND ELUT CERTIFY (1 ml), washed with 1 ml of 0.1 M acetic acid and 6 ml of methanol, and eluted with 2 ml of conc. HCl-methanol (9 : 91). The eluent was evaporated, redissolved in 1 ml of water, basified with 1 N NaOH and extracted with chloroform. The amphetamines in chloroform layer was derivatized with GITC at room temperature for 30 min. The GITC-derivatives were analysed by HPLC with a UV detector (254 nm). As a result, administration of l-DPN could be clearly discriminated from that of d-MA and dl-MA by comparisons of the enantiomeric compositions of MA excreted in the urine. Discrimination between administration of l-DPN and l-MA could be carried out by the apparent difference of ratio of AP/MA in the urine. The AP/MA ratio of the former was 10 times that of the latter.
公益社団法人日本薬学会の論文
1992-04-30