イオン会合体のサーモクロミズムによる製剤及び尿中のジブカインとプロカインの吸光光度定量

概要

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This paper deals with a selective spectrophotometric determination of dibucaine and procaine, local anesthetics, by using thermochromism of ion-associate. Tetrabromophenolphthalein ethyl ester (TBPE) is an excellent extraction reagent for quaternary ammonium ions and amines. TBPE forms red or blue ion-associates with amines or quaternary ammonium ions. Of these ion-associates, the red extract shows a reversible thermochromism. The rate of decrease of the absorbance with temperature change (ΔA/Δt) is proportional to the concentration of the amine in the aqueous phase (ΔA=A_<25℃>-A_<50℃>, Δt=50℃-25℃). Consequently, a straight line was obtained in the concentration range (1-5)×10^<-6>M dibucaine and procaine in the final aqueous solution, with ΔA/Δt as the ordinate and the concentration as the abscissa. Though the ΔA/Δt value of TBPE-dibucaine and -procaine associates were somewhat affected by ΔA/Δt of (5-10)×10^<-7>M each quaternary ammonium salt, a good recovery could be obtained by subtracting the ΔA/Δt value of each salt measured previously from the ΔA/Δt value of the mixture. This method is useful for the determination of amines added to the human urine.
公益社団法人日本薬学会の論文
1981-08-31