ベルベリンの溶媒抽出による比色定量法

概要

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Berberine is extracted into 1,2-dichloroethane as a 1:1 ion-pair with tetrabromophenolphthalein ethyl ester (TBPE). The proposed method has high sensitivity and can be applied to the determination of berberine in samples contahing tannate. The inorganic substances, such as sodium chloride, calcium chloride and ammonium sulfate, as well as tannate of 10000 fold molar excess over berberine had no influence on the absorbance of the extract. Amines, such as thiamine and papaverine of (5〜10) fold molar excess interfered slightly. The calibration curve was prepared as follows; (1〜10) ml of a standard berberine solution (1x1O_<->5M) is taken in the separatory funnel, and 5 ml of borate (O.1M)-phosphate (O.3M) buffer (pH8.5) and 2ml of TBPE ethanol solution (4×10_<-3>M) are added. It is diluted to 50ml with water, and shaken with 10ml of 1,2-dichloroethane for 3 min. The extract is filtered through a filter paper to remove droplets of water. The absorbance is measured at 610 nm against a reagent blank.
社団法人日本分析化学会の論文
1975-02-10