2-ニトロソ-5-(N-エチル-N-スルホプロピルアミノ)フェノールを用いたフローインジェクション法による微量鉄の定量

概要

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Spectrophotometric determination of trace amount! of iron by flow injection analysis was investigated. Iron (II) forms a water-soluble chelate with 2-nitroso-5 -(A^ethyl-A^-sulfopropyl-amino)phenol (nitroso-ESAP). Carrier solution (2.0 × 10^<-4>M ascorbic acid in buffet solution, pH 7) and reagent solution (1.5 × 10^<-4>M nitroso-ESAP in buffer solution, pH 7) were propelled by double plunger pump (flow rate, Pl : 1.4ml/min, P_2 : 1.2 ml/min), and a sample solution (I50μl) was injected into a carrier stream via a loopvalve sample injector. The carrier stream met a reagenl stream and flowed together into a reaction coil (0.5 mm i.d. × 10 m). The absorbance was measured at 752 nm. Galcium(II), cadmium(II), magnesium(II), aluminium (III), zinc(II), mercury(II), silver(I), lead(II), and nickel(II) did not interfere with determination of iron. Ghromium(III) and copper(II) gave an interference, but the interference of copper was depressed by addition of ammonium buffer solution in reagent solution. Sampling rate was 20 samples per h. The relative standard deviation was about 0.5 %. The flow injection system could be applied to the determination of total iron in water and serum samples.
社団法人日本分析化学会の論文
1984-06-05