ペンタクロロフェノールの代謝に伴う活性酸素種の発生と脂質過酸化反応

概要

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Pentachlorophenol (PCP), used as an organochlorine herbicide, has toxicity related with active oxygen species such as the strand break of DNA possibility. It was recognized by the thiobarbituric acid (TBA) method and the oxygen electrode method that active oxygen species, superoxide and hydroxyl radicals, were produced with the metabolism of PCP in a microsomal system in vitro. The TBA-reactive substance (TBARS) content in the reaction mixture and the rate of oxygen uptake were correlatively increased by the addition of PCP. Moreover, the liver weight, the TBARS content and GSSG content in the liver of mice increased and flavin content in the liver and the enzyme activities in the liver cytosol of mice decreased after the administration of PCP. Each parameter was correlatively changed by the dose of PCP. We considered that the active oxygen species, superoxide and hydroxyl radicals, were produced with the metabolism of PCP in microsome and the lipid peroxidation was related with the hepatic toxicity of PCP in vivo.
社団法人日本薬学会の論文
1993-12-31