Simultaneous Determination of Glycyrrhizin, Glycyrrhetic Acid and Glycyrrhetic Acid Mono-Glucuronide in Shakuyaku-kanzo-to Incubated with Rat Feces by Semi-micro High-Performance Liquid Chromatography

概要

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A method for semi-micro high-performance liquid chromatography(HPLC)has been established for the simultaneous determination of glycyrrhizin(GL), glycyrrhetic acid(GA)and glycyrrhetic acid mono-glucuronide(GAMG)in incubation mixtures of rat feces with Shakuyaku-kanzo-to decoction(combination of licorice root and peony root). The analysis could be accomplished within 20min with a TSKgel ODS-80TsQA(l50×2.0mm i.d.)column by linear gradient elution using a mobile phase containing aqueous phosphoric acid and acetonitrile at a flow rate of 0.2 ml・min^<-1> a thermostatic oven at 25℃, and detection at 254 nm. The detection limits of these compounds were 0.1-0.85 pmol per injection(5μl). The concentrations of GL and its metabolites in the incubation mixture after continuous consumption of Shakuyaku-kanzo-to were significantly different compared with those of untreated control. GL-hydrolysis of rat feces was enhanced by pre-consumption of Shakuyaku-kanzo-to.
社団法人日本薬学会の論文
2001-10-01