Simultaneous Determination of Principal Ingredients in Kampo Medicines by High-Performance Liquid Chromatography

概要

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A simultaneous high-performance liquid chromatographic method for the determination of sennoside A, aloe-emodin, rhein, emodin, chrysophanol, glycyrrhizin, paeoniflorin, naringin, neohesperidin, baicalin, wogonoside, cinnamaldehyde, magnolol and honokiol in Kampo medicines containing rhubarb as the main constituent crude drug was established. The analyses were performed with a Wakosil-II 5C18HG column by linear gradient elution using a mobile phase containing aqueous phosphoric acid and acetonitrile at a flow rate of 1.0 ml・min^<-1>, a thermostatic oven at 35℃, and detection at 265 nm. The detection limits of these compounds were 0.3-8.0 pmol per injection (5 μl). This method was applied to determine the quantities in Kampo decoctions; Daio-kanzo-to, Choi-joki-to, Sho-joki-to, Mashinin-gan, Dai-saiko-to, Keisi-ka-shakuyaku-daio-to and Tokaku-joki-to. The within-day relative standard deviations were less than 2.52% (n = 10). The recoveries of these compounds were from 81.5 to 107%. The fourteen marker components in seven kinds of Kampo medicines were easily determined within 40 min without interference from co-existing components.
2002-02-20