Antifungal Pterocarpan Derivatives

概要

論文の詳細を見る
l-Maackiain inhibits the growth of Pythium graminicola and P. vanterpooli at lower doses when tested by incorporation into the culture media than when determined by the usual MIC method. To solve this problem, the stability of l-maackiain was examined in the incubation mixture and in acid solution at elevated temperatures. In the acid hydrolysate, 2',7-dihydroxy- 4',5'-methylenedioxyisoflav-3-ene (compound 1) and 2-(2',4'-dihydroxyphenyl)-3-methyl-5,6- methyienedioxybenzofuran (compound 2) were identified. Neither of compounds 1 and 2 was found among the metabolites of P. graminicola and P. vanterpooli, but compound 1 was identified in the sterilized cultures. Compounds 1 and 2 inhibited the growth of P. graminicola at concentrations of 20 and 10 μg/ml, respectively, whereas l-maackiain itself inhibited the growth at a concentration of 50 μg/ml. Compounds 1, 2 and l-maackiain inhibited the growth of P. vanterpooli at a concentration of 20 μg/ml. These findings indicated that the possible participation of compound 1, produced in the sterilization process, in the inhibition of growth of P. graminicola by l-maackiain.
日本生薬学会の論文
1993-06-20