The Inactivation Profile of Rabbit Muscle Creatine Phosphokinase in Tris-acetate Buffer Solutions
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概要
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The inactivation profile of rabbit muscle creatine phosphokinase (CPK) has been investigated in Tris-acetate buffer solutions under various conditions of pH and temperature. The inactivation pattern follows apparent first-order kinetics at pH very close to 6.00 (isoelectric point of CPK) and 30 to 39℃ and at pH 7.40,39℃. The activation energy of the inactivation at pH very close to 6.00 is 31.8 kcal/mol. The inactivation half-lives of CPK are 13.6 h (pH 5.95,30℃), 7.9 h (pH 5.93,35℃), 3.0 h (pH 5.90,39℃) and 11.9 h (pH 7.40,39℃). At pH 6.70,the inactivation profile follows a biexponential curve at 30℃ and at 39℃. The time course consists of two types of inactivation : an initial rapid inactivation (α-phase) and a gradual inactivation (β-phase). Anomalous irregular inactivation profiles are also observed at pH 7.00,7.40 and 8.00 at 30℃ as well as at pH 8.00 at 39℃. Some possible explanations of these irregular inactivation profiles are presented.
- 公益社団法人日本薬学会の論文
- 1981-11-25
著者
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渡辺 淳
Department of Biopharmaceutics, Faculty of Pharmaceutical Science, Nagoya City University
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渡辺 淳
Department Of Biopharmaceutics Faculty Of Pharmaceutical Sciences Nagoya City University
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許 興智
Department of Pharmaceutics, Chia Nan Jr. College of Pharmacy
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許 興智
Department Of Pharmaceutics Chia Nan Jr. College Of Pharmacy
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